Modifying Enzymes
Worthington Biochemical Corporation PAPAIN DISSOCIATION SYSTEM
Papain Dissociation System, 100mL of EBBS, 2degree to 8degreeC Storage Temp, 1BX
Sigma Aldrich Fine Chemicals Biosciences Benzonase(R) endonuclease EMPROVE(R) Expert | 9025-65-4 | MFCD00131010 |
Benzonase(R) endonuclease EMPROVE(R) Expert | 9025-65-4 | MFCD00131010 |
Sigma Aldrich Fine Chemicals Biosciences Neuraminidase (Sialidase) from Arthrobacter ureafaciens |
Neuraminidase (Sialidase) from Arthrobacter ureafaciens |
Sigma Aldrich Fine Chemicals Biosciences Amyloglucosidase from Aspergillus niger lyophilized powder, 30-60 units/mg protein (biuret), <=0.02% glucose | 9032-08-0 | MFCD00081350 | 100MG
Amyloglucosidase from Aspergillus niger lyophilized powder, 30-60 units/mg protein (biuret), <=0.02% glucose | 9032-08-0 | MFCD00081350 | 100MG
Sigma Aldrich Fine Chemicals Biosciences DNase I recombinant, 2 x 10000 units
DNase I, recombinant, grade I is used for: Eliminating DNA during protein isolation procedures. Analysis of chromatin structure. Eliminating DNA during sample preparation.
Worthington Biochemical Corporation Trypsin, TPCK Treated, 100mg
A chromatographically purified, diafiltered, lyophilized powder that has been treated with L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK) to inhibit contaminating chymotryptic activity [Kostka, V., and Carpenter, F.: JBC, 239, 1799 (1964)]. Store at 2-8°C. Protect from moisture.
New England Biolabs, Inc. Rapid™ PNGase F – 50 reactions
Rapid PNGase F enables complete and rapid deglycosylation of antibodies and immunoglobulin fusion proteins, as well as other glycoproteins, in minutes. All N-glycans are released rapidly and without bias, and are ready to be prepared for downstream chromatography or mass spectrometry analysis. Rapid PNGase F creates an optimized workflow, reducing processing time without compromising sensitivity or reproducibility.
- Complete deglycosylation of antibodies and immunoglobulin fusion proteins in 10 minutes
- Recombinant enzyme; guaranteed endoglycosidase F1, F2 or F3 free
- All reaction components are compatible with HPLC and mass spectrometry analysis
- >= 95% purity, as determined by SDS-PAGE and intact ESI-MS
- Optimal activity and stability ensured for up to 12 months
Worthington Biochemical Corporation MICROCOCCUS LYSODEIKTICUS LYO
Dried cells. Suitable lysozyme substrate. 5gm. Store at 2-8�C.
New England Biolabs, Inc. T3 DNA Ligase – 100000 units
T3 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T3. It will catalyze the formation of a phosphodiester bond between adjacent 5 phosphate and 3 hydroxyl groups of duplex DNA. Cohesive ends, blunt ends, and nick sealing can all be efficiently catalyzed by T3 DNA Ligase. As with T4 DNA Ligase, blunt end ligation is enhanced by the addition of PEG 6000 to the reaction. T3 DNA Ligase exhibits a higher tolerance (2-fold) for NaCl in the reaction compared to T4 DNA Ligase, making the enzyme a versatile choice for in vitro molecular biology protocols requiring DNA ligase activity.
New England Biolabs, Inc. ProtoScript II Reverse Transcriptase – 4000 units
ProtoScript II Reverse Transcriptase is a recombinant M-MuLV reverse transcriptase with reduced RNase H activity and increased thermostability. It can be used to synthesize first strand cDNA at higher temperatures than the wild type M-MuLV. The enzyme is active up to 48C, providing higher specificity, higher yield of cDNA and more full-length cDNA product up to 12 kb.
Gold Biotechnology Inc DNase I, Bovine Pancreas (Lysate Tested), >2000 Kunitz U/mg 100 mg
Bovine pancreatic deoxyribonuclease (DNase) is an endonuclease, which splits phosphodiester linkages preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with a free hydroxyl group at the 3' position and a phosphate group at the 5' position. The optimum pH is 7.8.
Sigma Aldrich Fine Chemicals Biosciences DNase I recombinant, RNase-free, 10000 units
DNase I recombinant, RNase-free may be used to degrade DNA in applications that are sensitive to the presence of Rnase.
New England Biolabs, Inc. Template Switching RT Enzyme Mix – 20 reactions
The reverse transcriptase (RT) in the Template Switching RT Enzyme Mix adds a few non-templated nucleotides after it reaches the 5' end of the RNA template. These non-templated nucleotides can anneal to a template switching oligo (TSO) with a known sequence, prompting the reverse transcriptase to switch template from RNA to the TSO. The resulting cDNA contains a known sequence (complementary to the sequence of the TSO) attached to the 3' end. This feature can be utilized in a variety of downstream applications, such as cDNA amplification, 5' RACE (rapid amplification of cDNA ends), and 2nd strand cDNA synthesis.
Discovery Life Sciences HUMAN CYP1A2+REDUCTASE 0.5NMOL
Human CYP1A2 + P450 Reductase SUPERSOMES 0.5 nmole cytochrome P450 in 0.5mL This activity is catalyzed by CYP1A2 which is expressed from human CYP1A2 cDNA using a baculovirus expression system